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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Monoclonal Antibodies Against Male-Specific Antigen of White Lamphun Cattle (Bos indicus)) ผู้เขียน: Sirikron Dumrongsri, Petai Pongpiachan, Supamit Mekchay สื่อสิ่งพิมพ์:pdf Abstractsperm. The objective of this study was to produce a monoclonal antibody against male-specific antigen of White Lamphun cattle. From this study, monoclonal antibodies were produced by biweekly immunization of 3 Balb/c mice with 10 ? 106 cells of White Lamphun cattle sperm. Mouse number 2 was chosen to be used in the fusion process by fusing myeloma cells and splenocytes from the immunized Balb/c female mouse with a ratio of 3:1 (splenocyte:myeloma) and the result from the enzyme-linked immunosorbent assay (ELISA) showed that hybridoma cells named P1C2 produced antibody amounts against male and female white blood cells of White Lamphun cattle of 0.55 and 0.38 optical density (O.D.) units. P1C2 was used in the cell limiting process, evaluated using antibody titer and resulted in positive clones named P1C2B8 and P1C2C9. The ELISA results showed that P1C2B8, P1C2C9 and P1C2B8&C9 produced antibody amounts against male and female white blood cells of 1.74 ? 0.03 and 1.56 ? 0.05, 1.04 ? 0.03 and 0.88 ? 0.03 and 1.47 ? 0.07 and 1.27 ? 0.07 O.D. units, respectively. The results from immunofluorescence showed that P1C2B8, P1C2C9 and P1C2B8&C9 produced antibody amounts against male and female white blood cells of 92.15 ? 0.87 and 69.33 ? 0.93, 91.19 ? 0.95 and 72.95 ? 1.27 and 93.27 ? 1.25 and 68.68 ? 1.23 %, respectively (P<0.05). It was concluded that the monoclonal antibodies produced in this study were specific to the male more than the female. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Relationships between Pectoralis Muscle Proteomes and Shear Force in Thai Indigenous Chicken Meat) ผู้เขียน:Tawatchai Teltathum, Supamit Mekchay สื่อสิ่งพิมพ์:pdf AbstractTwo-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF/MS) were used to investigate the association of pectoralis muscle proteomes with Warner-Bratzler shear force (WBSF) values in Thai indigenous chicken meat. A total of 169 proteome spots were found in chicken muscle. Of these, three protein spots were significantly upregulated and associated with high-WBSF values. These protein spots were characterized and showed homology with pyruvate kinase (PKM2), phosphoglycerate mutase1 (PGAM1) and triosephosphate isomerase1 (TPI1). Moreover, the protein expression levels were highly correlated to the WBSF values. The PKM2 and TPI1 proteins were positively correlated to the WBSF values (r=0.71, p<0.05 and r=0.65, p<0.05, respectively), whereas, the PGAM1 protein trended toward an association with the WBSF values (r=0.49, p=0.15). Additionally, the expression levels of PGAM1 were positively correlated to the TPI1 protein expression levels (r=0.88, p<0.01), whereas no significant correlation between the expression levels of PKM2 and PGAM1 (r=0.43, p=0.21) and PKM2 and TPI1 were found (r=0.51, p=0.13). The results indicated that these three proteomes of the glycolytic pathway are important in the energy metabolism processes of muscle. This finding promotes PKM2, PGAM1 and TPI1 as the functional protein markers for the tenderness trait in Thai indigenous chicken. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Genetic Polymorphism of Bovine Growth Hormone Gene on Preweaning Growth Traits in a Thai Multibreed Beef Population) ผู้เขียน:ชินา สุภากรณ์, Supamit Mekchay,  ดร.วรวิทย์ สิริพลวัฒน์, รองศาสตราจารย์ , ดร.พรรณวดี โสพรรณรัตน์, รองศาสตราจารย์ , ดร.ศกร คุณวุฒิฤทธิรณ, รองศาสตราจารย์ , ดร.ศรเทพ ธัมวาสร, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractGrowth hormone (GH) gene was investigated as a candidate gene for birth weight (BW) and weaning weight (WW) in a population of multibreed beef cattle of three crossbreds. Beef cattle involved consisted of Charolais, Brahman and Thai local native with different breed fractions. Screening polymorphisms within bovine GH gene was performed by using a single strand conformation polymorphism (SSCP) method and the result was confirmed by a sequencing analysis. To identify polymorphic sites within the bovine GH gene, primers were designed revealing 6 PCR fragments (GH1 to GH6). Four single nucleotide polymorphisms (SNPs) were found in the bovine GH gene of this cattle population. Two SNPs were found within the GH1 and GH2 fragments at nucleotide position 303 and 218 bp. The other two SNPs were located at position 208 and 388 bp of the GH5 fragment. No polymorphisms were found in the GH3, GH4 and GH6 fragments, respectively. The genotypic frequencies of GH1 were found to be 5.40, 85.40 and 9.20 percents for A1A1 A1B1 and B1B1, respectively. The genotypic frequencies of GH2 were found to be 90.00, 8.46 and 1.54 percents for A2A2, A2B2 and A2C2, respectively. The GH5 were found to be 2.31, 30.77, 17.69, 13.85 and 35.38 percents for A5A5, A5B5, B5C5, B5D5 and C5D5 genotypes, respectively. Considering haplotype of four polymorphic sites, only 17 haplotypes were identified in this population. The 212 and 215 haplotypes were found at a high frequency. Effect of four SNPs in each region genotype regions of GH for BW and WW indicated that GH1 influenced both BW and WW. However, the GH5 influenced only for BW (P<0.01). Animals with B1B1 of GH1 had the highest BW and WW but animals with A1A1 had the lowest of both traits (P<0.05). Animals with C5D5 of GH5 had the highest BW but animals with A5A5 had lowest BW (P<0.05). Moreover, haplotype of four SNPs were analysed and the results showed the animals with 313 and 325 haplotypes obtained high BW and WW, respectively (P<0.05). However, animals with 111 and 234 haplotypes obtained low BW and WW, respectively (P<0.05). Therefore, the producers should select animals with B1B1 and C5D5 genotypes to obtain high BW and WW. Moreover, mating system should be considered because intra- and inter-genic polymorphic regions or haplotypes has an effect on BW and WW. |
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